TLR4 Polyclonal Antibody

• Background :

  Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:9237759, PubMed:10835634) . Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific (PubMed:20711192) . Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression (PubMed:15809303) . In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187) .

• Synonyms :

  Toll-like receptor 4; TLR4; CD284

• Gene ID :

  7099

• Swiss Prot :

  O00206

• Cellular Locus :

  Cell membrane

• Host :

  Rabbit

• Cross Reactivity :

  Human

• Immunogen :

  121-200/839

• Target :

  TLR4

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic peptide derived from human TLR4

• Applications :

  WB, ELISA, FCM, IHC-P

• Purification :

  Purified by Protein A.

• Concentration :

  1µg/µl

• Dilution :

  WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Specificity :

  Mouse TLR4 has 75% homology to the antibody immunogen sequence

• Gene ID URL :

  7099

• Predicted Cross Reactivity :

  Mouse, Rat, Cow, Sheep, Pig