MAML1 Polyclonal Antibody

• Background :

  Notch receptors are involved in cell-fate determination in organisms as diverse as flies, frogs, and humans (1) . The 'mastermind' gene has been identified in multiple genetic screens for modifiers of Notch mutations in Drosophila melanogaster (2) . In Drosophila, loss-of-function mutations of Notch produce a 'neurogenic' phenotype in which cells destined to become epidermis switch fate and differentiate to neural cells (2) . The human homolog, mastermind-like 1 (Mam1), localizes to nuclear bodies (2-4) . Mam1 binds to the ankyrin repeat domain of all four mammalian Notch receptors, forms a DNA-binding complex with ICN and RBP-Jk, and amplifies Notch-induced transcription of Hes1 (2) . Mam1 is an essential component of the transcriptional apparatus of Notch signaling (5) . The gene which encodes Mam1 maps to human chromosome 5 (4) .

• Synonyms :

  Mam-1; Mam1; MAML 1; MAML 1; Maml1; MAML1_HUMAN; Mastermind like 1; Mastermind-like protein 1; mKIAA0200.

• Gene ID :

  9794

• Cellular Locus :

  Nucleus

• Host :

  Rabbit

• Cross Reactivity :

  Human

• Immunogen :

  401-500/1016

• Target :

  MAML1

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic peptide derived from human MAML1

• Applications :

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification :

  Purified by Protein A.

• Concentration :

  1µg/µl

• Dilution :

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL :

  9794

• Predicted Cross Reactivity :

  Mouse, Rat, Dog, Horse