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Amisyn Polyclonal Antibody

CAT: 0498-bs-11039RSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-11039RSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Amisyn is a mostly cytosolic protein related to Tomosyn which plays an important role in SNARE complex assembly. Amisyn contains a v-SNARE coiled coil homology domain that binds to Syntaxin 1A and weakly to Syntaxin 4. Three isoforms exist for Amisyn. Isoform 1 is the full length protein, isoform 2 has a different amino acid sequence between residues 204-210 and isoform 3 is missing amino acids 1-102 and contains a different sequence for amino acids 103-150. Amisyn lacks a transmembrane domain and therefore is unable to assemble into a functional, membrane-anchored SNARE complex. This suggests that Amisyn may instead be acting to maintain SNARE conformation and facilitate the binding of VAMP-2. Amisyn can inhibit exocytosis independent of Syntaxin binding.
Synonyms
Amisyn; HSPC156; STXB6_HUMAN; STXBP6; Syntaxin binding protein 6; Syntaxin-binding protein 6.
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Immunogen
1-100/210
Target
Amisyn
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human Amisyn
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Predicted Cross Reactivity
Human, Mouse, Rat, Cow, Sheep, Chicken, Rabbit

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