CD163 Polyclonal Antibody

• Background:

  Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

• Synonyms:

  Scavenger receptor cysteine-rich type 1 protein M130; CD163; sCD163; M130

• Gene ID:

  9332

• Swiss Prot:

  Q86VB7

• Cellular Locus:

  Secreted, Cell membrane

• Host:

  Rabbit

• Cross Reactivity:

  Human

• Immunogen:

  101-200/1156

• Target:

  CD163

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human CD163.

• Applications:

  WB, ELISA, FCM, IHC-P

• Purification:

  Purified by Protein A.

• Concentration:

  1ug/ul

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Prediction Reactivity:

  Mouse, Rat

• Gene ID URL:

  9332

• Isotype:

  IgG