PHD3 Polyclonal Antibody

• Background :

  PHD3 (Egl nine homolog 3; Hypoxia-inducible factor prolyl hydroxylase 3; HIF-prolyl hydroxylase 3; HIF-PH3; HPH-1; Egln3; Prolyl hydroxylase domain-containing protein 3; ) Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism. [Catalytic Activity] An HIF alpha chain L-proline + 2-oxoglutarate+ O (2) = An HIF alpha chain trans-4-hydroxy-L-proline + succinate + CO (2) . [Subcellular Location] Cytoplasm. Nucleus. Widely expressed at low levels. Expressed athigher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.

• Synonyms :

  EGL9 homolog of C. elegans 3; EGLN3; Factor responsive smooth muscle protein; H Prolyl Hydroxylase 3; HP4H3; HPH3; P4H3; Prolyl Hydroxylase Domain Containing Protein 3; SM20; Hypoxia-inducible factor prolyl hydroxylase 3; HIF-PH3; HPH-3.

• Gene ID :

  112399

• Swiss Prot :

  Q9H6Z9

• Cellular Locus :

  Nucleus

• Host :

  Rabbit

• Cross Reactivity :

  Mouse

• Immunogen :

  151-239/239

• Target :

  PHD3

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic peptide derived from PHD3

• Applications :

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification :

  Purified by Protein A.

• Concentration :

  1ug/ul

• Dilution :

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Prediction Reactivity :

  Human, Rat, Dog, Cow, Pig

• Gene ID URL :

  112399

• Isotype :

  IgG