SYNC Antibody / Syncoilin

CAT: 0800-FY12115Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-FY12115Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
Q9H7C4
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human Syncoilin/SYNC recombinant protein (Position: D11-Q479) was used as the immunogen for the SYNC antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, IF, ICC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This SYNC antibody is available for research use only.
Storage Conditions
After reconstitution, the SYNC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the SYNC antibody should be determined by the researcher.
Image Legend
IF analysis of Syncoilin/SYNC using anti-SYNC antibody (red) . SYNC was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SYNC antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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