CD300A Antibody

• Specifications:

  Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q9UGN4

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  E.coli-derived human CD300A recombinant protein (Position: L156-T299) was used as the immunogen for the CD300A antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, ELISA

• Purity:

  Immunogen affinity purified

• Format:

  Lyophilized

• Buffer:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Limitations:

  This CD300A antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD300A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

• Applications Notes:

  Optimal dilution of the CD300A antibody should be determined by the researcher.

• Image Legend:

  Flow Cytometry analysis of U20S cells using anti-CD300A antibody. Overlay histogram showing U20S cells stained with (Blue line) . The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD300A antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.