Products for Research Use Only
RegisterLogin

FHIT Antibody

CAT: 0800-RQ4518Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-RQ4518Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Bis (5'-adenosyl) -triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1, P3-bis (5'-adenosyl) -triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P49789
Host
Mouse
Reactivity
Human
Immunogen
Human FHIT recombinant protein (amino acids M1-Q147) was used as the immunogen for the FHIT antibody antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
26H7
Applications
WB, IF, FACS
Purity
Protein G affinity
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Limitations
This FHIT antibody is available for research use only.
Storage Conditions
After reconstitution, the FHIT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the FHIT antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
Immunofluorescent staining of FFPE human MCF7 cells with FHIT antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P49789

Bis(5'-adenosyl)-triphosphatase

FHIT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P49789
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
FHIT
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.6.1.29
Processing
—
Secondary accessions
A2IAS9, A2IAT0, A2IAT6, A8K1A9, Q45QG9, Q6IU12
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

ApoptosisTranscriptionTranscription regulation

Coding sequence diversity

Chromosomal rearrangement

Cellular component

CytoplasmMitochondrionNucleus

Molecular function

HydrolaseTransferase

Ligand

ManganeseNucleotide-binding

PTM

Phosphoprotein

Disease

Tumor suppressor

Related Products

CatalogName
RQ4624NONO Antibody / p54nrb Antibody
F53952-0.2MLAntibody
F53952-0.05MLAntibody
F53954-0.2MLAntibody
F53954-0.05MLAntibody
V8674SAF-100UGInhibin alpha Antibody / INHA antibody
V8674-100UGInhibin alpha Antibody / INHA antibody
V8674-20UGInhibin alpha Antibody / INHA antibody
R20161SAF-250UGLambda Light Chain Antibody / Lambda Antibody
R30971B-Actin Antibody / Beta Actin Antibody

Alternative Products

CatalogName
GWB-270694FHIT, Antibody
70R-49744FHIT antibody

Popular Products