LEO1 Antibody / RNA polymerase-associated protein LEO1

• Description:

  RNA polymerase-associated protein LEO1 is an enzyme that in humans is encoded by the LEO1 gene. LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q8WVC0

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An E.coli-derived human recombinant protein (amino acids E355-R593) was used as the immunogen for the LEO1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, IF, FACS, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Limitations:

  This LEO1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the LEO1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the LEO1 antibody should be determined by the researcher.

• Location:

  Nuclear

• Image Legend:

  Immunofluorescent staining of FFPE human HeLa cells with LEO1 antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.