DNA Polymerase lambda Antibody / POLL

• Description :

  DNA polymerase lambda, also known as POLL, is a protein found in eukaryotes. In humans, it is encoded by the POLLA gene. POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, suggesting that POLL participates in short- and long-patch base excision repair. POLL is widely expressed, with abundant expression in pachytene spermatocytes of testis and in ovary.

• Specifications :

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  Q9UGP5

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A recombinant human protein corresponding to amino acids K307-N407 was used as the immunogen for the DNA Polymerase lambda antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, FACS, ELISA

• Purity :

  Antigen affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This DNA Polymerase lambda antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the DNA Polymerase lambda antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic

• Image Legend :

  Western blot testing of human 1) HeLa, 2) COLO-320, 3) MCF7 and 4) HepG2 cell lysate with DNA Polymerase lambda antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa, also observed at ~68 kDa.