DNA Polymerase lambda Antibody / POLL

CAT: 0800-RQ4359Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ4359Size:100 µg
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Description
DNA polymerase lambda, also known as POLL, is a protein found in eukaryotes. In humans, it is encoded by the POLLA gene. POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, suggesting that POLL participates in short- and long-patch base excision repair. POLL is widely expressed, with abundant expression in pachytene spermatocytes of testis and in ovary.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9UGP5
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human protein corresponding to amino acids K307-N407 was used as the immunogen for the DNA Polymerase lambda antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This DNA Polymerase lambda antibody is available for research use only.
Storage Conditions
After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the DNA Polymerase lambda antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human 1) HeLa, 2) COLO-320, 3) MCF7 and 4) HepG2 cell lysate with DNA Polymerase lambda antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa, also observed at ~68 kDa.

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