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Intersectin 1 Antibody / ITSN1

CAT: 0800-RQ8715Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8715Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Intersectin-1 is a protein that, in humans, is encoded by the ITSN1 gene. The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
Q15811
Host
Rabbit
Reactivity
Human
Immunogen
An E.coli-derived human recombinant protein (amino acids A522-P1721) was used as the immunogen for the Intersectin 1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, ELISA, IHC-P
Purity
Antigen affinity chromatography
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Limitations
This Intersectin 1 antibody is available for research use only.
Storage Conditions
After reconstitution, the Intersectin 1 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Intersectin 1 antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human brain tissue with Intersectin 1 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · Q15811

Intersectin-1

ITSN1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15811
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITSN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A7Y322, A8CTX8, A8CTY3, A8CTY7, A8D7D0, A8DCP3, B4DTM2, E7ERJ1, E9PE44, E9PG01, E9PHV2, O95216, Q0PW94, Q0PW95, Q0PW97, Q14BD3, Q1ED40, Q20BK3, Q9UET5, Q9UK60, Q9UNK1, Q9UNK2, Q9UQ92
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Cellular component

Cell membraneCell projectionCoated pitCytoplasmCytoplasmic vesicleEndosomeMembraneNucleusSynapseSynaptosome

Domain

Coiled coilRepeatSH3 domain

Biological process

EndocytosisExocytosisHost-virus interactionProtein transportTransport

PTM

Phosphoprotein

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