INTS12 Antibody / Integrator complex subunit 12

• Description:

  Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. [UniProt]

• Specifications:

  Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q96CB8

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An E.coli-derived human recombinant protein (amino acids M1-K384) was used as the immunogen for the INTS12 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, ELISA

• Purity:

  Antigen affinity chromatography

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Limitations:

  This INTS12 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the INTS12 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the INTS12 antibody should be determined by the researcher.

• Image Legend:

  Flow cytometry testing of fixed and permeabilized human MCF7 cells with INTS12 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= INTS12 antibody.