Zebrafish Her4 Antibody / Her4.1 / Her4.2 / Her4.3 / Her4.5

• Description:

  Her4 is prominently expressed in the developing central nervous system, where it maintains neural progenitor populations by inhibiting premature neuronal differentiation. By repressing proneural gene expression, Her4 ensures the proper timing and patterning of neuron formation. It also contributes to lateral inhibition, a process critical for the spatial organization of neural tissues. Because of its well-characterized role in Notch-mediated signaling and neurodevelopment, zebrafish Her4 is frequently used as a molecular marker for neural progenitor cells and as a model to study stem cell maintenance, differentiation, and the impact of disrupted Notch signaling in developmental disorders and disease.

• Specifications:

  Immunohistochemistry (FFPE) : 2-5 µg/mL

• UniProt:

  Q90466

• Host:

  Rabbit

• Reactivity:

  Zebrafish

• Immunogen:

  E. coli-derived zebrafish Her4 recombinant protein (amino acids M1-W152) was used as the immunogen for the Zebrafish Her4 antibody. This antibody will detect isoforms 1 & 2 & 3 & 5.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC-P

• Purity:

  Antigen affinity chromatography

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Limitations:

  This Zebrafish Her4 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Zebrafish Her4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Zebrafish Her4 antibody should be determined by the researcher.

• Location:

  Nuclear

• Image Legend:

  IHC staining of zebrafish Her4 protein using Zebrafish Her4 antibody, HRP-labeled secondary and DAB substrate. Her4 was detected in a paraffin-embedded section of zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.