Histone Antibody / Histone H1

• Description:

  Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

• UniProt:

  P07305

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  AE-4

• Applications:

  FACS, IF, IHC-P, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the Histone antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Histone antibody is available for research use only.

• Storage Conditions:

  Store the Histone antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Histone H1 antibody (AE-4)