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P53 (pS392) Antibody

CAT: 0013-GTR17856864Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR17856864Size:0.1 mg
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Description
Mouse monoclonal antibody to p53 (phospho-Ser392)
Product Name Alternative
BCC7, TRP53, TP53, LFS1
UniProt
P04637
Reactivity
Human
Immunogen
KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.
Target
P53 (pS392)
Clonality
Monoclonal
Clone
FP3.2 [FPS392]
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunohistochemistry (paraffin sections) : Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10 μg/ml, incubation: 1 hour at RT; or overnight at 4°C, positive tissue: breast carcinoma with high level of wild-type p53. Western blotting: recommended dilution: 1 μg/ml.
Tested Applications
IHC-P, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG1
Entrez
7157

UniProtKB · P04637

Cellular tumor antigen p53

P53_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04637
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TP53
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q15086, Q15087, Q15088, Q16535, Q16807, Q16808, Q16809, Q16810, Q16811, Q16848, Q2XN98, Q3LRW1, Q3LRW2, Q3LRW3, Q3LRW4, Q3LRW5, Q86UG1, Q8J016, Q99659, Q9BTM4, Q9HAQ8, Q9NP68, Q9NPJ2, Q9NZD0, Q9UBI2, Q9UQ61
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDNA-bindingRepressor

Coding sequence diversity

Alternative promoter usageAlternative splicing

Biological process

ApoptosisBiological rhythmsCell cycleHost-virus interactionNecrosisTranscriptionTranscription regulation

Cellular component

CytoplasmCytoskeletonEndoplasmic reticulumMitochondrionNucleus

Disease

Disease variantLi-Fraumeni syndromeTumor suppressor

Ligand

Metal-bindingZinc

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