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HLA-G Antibody

CAT: 0013-GTR17856798Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR17856798Size:0.1 mg
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Description
Mouse Monoclonal to HLA-G.
UniProt
P17693
Reactivity
Human
Immunogen
C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
Target
HLA-G
Clonality
Monoclonal
Clone
5A6G7
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. Immunohistochemistry (frozen sections) : Recommended dilution: 10 μg/ml; positive tissue: placenta.Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: placenta. ELISA: Positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant; negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. orb44577) . Western blotting: Positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE.
Tested Applications
ELISA, FC, ICC, IHC-Fr, IHC-P, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG1
Entrez
3135

UniProtKB · P17693

HLA class I histocompatibility antigen, alpha chain G

HLAG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P17693
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HLA-G
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCell projectionEndoplasmic reticulumEndosomeMembraneMHC ISecreted

PTM

Disulfide bondGlycoprotein

Biological process

Immunity

Domain

SignalTransmembraneTransmembrane helix

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