Human Amylin (Amylin) ELISA Kit

• Synonyms:

  IAPP; IAP; DAP; Islet Amyloid Polypeptide; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide

• Cross Reactivity:

  The kit detected Human Amylin in the samples and no significant cross-species reactions were found

• Target:

  Amylin

• Type:

  Colormetric

• Detection Method:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Amylin. During the reaction, Human Amylin in the sample or standard competes with a fixed amount of Human Amylin on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Amylin. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Amylin in tested samples can be calculated by comparing the OD of the samples to the standard curve.

• Assay Type:

  Competitive

• Assay Performance Time:

  1.5h

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  6.17-500pg/mL

• Precision:

  CV<10%

• Recovery:

  80%-120%

• Sensitivity:

  2.55pg/mL

• Storage Conditions:

  Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

• Species:

  Human