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Mouse FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit

CAT: 1444-EM23393Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1444-EM23393Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Synonyms
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
Cross Reactivity
The kit detected Mouse FGF2 in the samples and no significant cross-species reactions were found
Target
FGF2
Type
Colormetric
Detection Method
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse FGF2. During the reaction, Mouse FGF2 in the sample or standard competes with a fixed amount of Mouse FGF2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse FGF2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse FGF2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Type
Competitive
Assay Performance Time
1.5h
Sample Type
Serum, plasma and other biological fluids
Detection Range
12.35-1000pg/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
4.83pg/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Mouse

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