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Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

CAT: 1444-EH10185Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1444-EH10185Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Synonyms
FGF-2; B-FGF; BFGF; FGFB; HBGF-2; prostatropin; heparin-binding growth factor 2; FGF-β
UniProt
P09038
Cross Reactivity
The kit detected Human bFGF/FGF2 in the samples and no significant cross-species reactions were found
Target
BFGF/FGF2
Type
Colormetric
Field of Research
Cancer, Cardiovascular, Developmental biology, Neuroscience, Signal transduction, Stem cells
Detection Method
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human bFGF/FGF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human bFGF/FGF2. You can calculate the concentration of Human bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.
Assay Type
Sandwich
Assay Performance Time
3.25h
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000pg/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
18.75pg/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Human

UniProtKB · P09038

Fibroblast growth factor 2

FGF2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09038
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
FGF2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
FGF-2
EC number
—
Processing
Precursor
Secondary accessions
A4LBB8, O00527, P78443, Q16443, Q5PY50, Q7KZ11, Q7KZ72, Q9UC54, Q9UCS5, Q9UCS6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative initiation

Biological process

AngiogenesisDifferentiation

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

PTM

Isopeptide bondMethylationPhosphoproteinUbl conjugation

Cellular component

NucleusSecreted

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