Human E2 (Estradiol) ELISA Kit

• Synonyms:

  Estradiol; E2

• Cross Reactivity:

  The kit detected Human/Monkey/Mouse E2 in the samples and no significant cross-species reactions were found

• Target:

  E2

• Type:

  Colormetric

• Field of Research:

  Signal transduction, Neuroscience, Developmental biology

• Detection Method:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human/Monkey/Mouse E2. During the reaction, Human/Monkey/Mouse E2 in the sample or standard competes with a fixed amount of Human/Monkey/Mouse E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human/Monkey/Mouse E2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human/Monkey/Mouse E2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

• Assay Type:

  Competitive

• Assay Performance Time:

  1.5h

• Sample Type:

  Serum, Plasma, Urine, Saliva

• Detection Range:

  12.35-1000pg/mL

• Precision:

  CV<10%

• Recovery:

  80%-120%

• Sensitivity:

  4.93pg/mL

• Storage Conditions:

  Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

• Species:

  Human