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Human β-CTx (Beta Crosslaps) ELISA Kit

CAT: 1444-EH20208Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1444-EH20208Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Synonyms
B-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
UniProt
P02452 (COL1A1)
Cross Reactivity
The kit detected Human β-CTx in the samples and no significant cross-species reactions were found
Target
β-CTx
Type
Colormetric
Field of Research
Signal transduction, Metabolism
Detection Method
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human β-CTx. During the reaction, Human β-CTx in the sample or standard competes with a fixed amount of Human β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Type
Competitive
Assay Performance Time
1.5h
Sample Type
Serum, plasma and other biological fluids
Detection Range
125-8000pg/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
75pg/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Human

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