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Mouse β-CTx (Beta Crosslaps) ELISA Kit

CAT: 0763-E-EL-M0372-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-M0372-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
Β-CTx
UniProt
P11087 (COL1A1)
Cross Reactivity
This kit recognizes Mouse β-CTx in samples.No significant cross-reactivity or interference between Mouse β-CTx and analogues was observed
Target
B-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Signal transduction; Metabolism
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-CTx. During the reaction, Mouse β-CTx in the sample or standard competes with a fixed amount of Mouse β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
46.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://pubs.acs.org/doi/abs/10.1021/jacs.0c07309
Citation 02
https://www.sciencedirect.com/science/article/pii/S0753332222010113
Citation 03
https://www.nature.com/articles/s41419-020-02947-3
Citation 04
https://asbmr.onlinelibrary.wiley.com/doi/abs/10.1002/jbmr.4025
Citation 05
https://www.sciencedirect.com/science/article/pii/S1567576923002138
Citation 06
https://www.sciencedirect.com/science/article/pii/S0925443914001057
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse β-CTx concentrations in serum, plasma and other biological fluids.

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