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Human PAF (Platelet Activating Factor) ELISA Kit

CAT: 1444-EH10032Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1444-EH10032Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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UniProt
P80303 (25-106aa)
Cross Reactivity
The kit detected Human PAF in the samples and no significant cross-species reactions were found
Target
PAF
Type
Colormetric
Field of Research
Epigenetics and Nuclear Signaling, Biochemicals
Detection Method
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAF. You can calculate the concentration of Human PAF in the samples by comparing the OD of the samples to the standard curve.
Assay Type
Sandwich
Assay Performance Time
3.25h
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000pg/mL
Precision
CV<10%
Recovery
80%-120%
Sensitivity
46.88pg/mL
Storage Conditions
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
Species
Human

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