Cdc27 Rabbit Polyclonal Antibody

• Background :

  The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been iPTM:Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis., similarity:Belongs to the APC3/CDC27 family., similarity:Contains 9 TPR repeats., subunit:Interacts with RB.

• Product Name Alternative :

  CDC27; ANAPC3; D0S1430E; D17S978E; Cell division cycle protein 27 homolog; Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC

• Gene Name :

  CDC27

• Gene ID :

  996

• Swiss Prot :

  P30260

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC, ICC/IF, ELISA

• Field of Research :

  Cell_Cycle_G1S; Cell_Cycle_G2M_DNA; Oocyte meiosis; Ubiquitin mediated proteolysis; Progesterone-mediated oocyte maturation

• Purification :

  Affinity purification

• Dilution :

  WB 1:500-1:2000, IHC 1:100-1:300, ICC/IF 1:50-1:200, ELISA 1:5000-1:10000

• Form :

  Liquid

• Buffer :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

• Modification :

  Unmodified

• Molecular Weight :

  92kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype :

  IgG