NF-E4 (Acetyl Lys43) Rabbit Polyclonal Antibody

• Background:

  The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq, Jul 2008], function:Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR) . The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma-globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter., PTM:Acetylation at Lys-43 prolongs the protein half-life by preventing ubiquitin-mediated degradation and reduces the interaction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor at the gamma-promoter., PTM:Ubiquitinated; leading to its degradation by the proteasome. Acetylation at Lys-43 prevents ubiquitination., sequence caution:Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon., subunit:Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4. Interacts with HDAC1 and PCAF. Isoform 2 interacts with TFCP2., tissue specificity:Specifically expressed in fetal liver, cord blood and bone marrow. Also expressed in the K562 and HEL cell lines, which constitutively express the fetal globin genes.

• Product Name Alternative:

  NFE4; Transcription factor NF-E4

• Gene Name:

  NFE4

• Gene ID:

  58160

• Swiss Prot:

  Q86UQ8

• Host:

  Rabbit

• Reactivity:

  Human, Rat, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, ELISA

• Purification:

  Affinity purification

• Dilution:

  WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:2000-1:20000

• Form:

  Liquid

• Buffer:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

• Modification:

  Acetylated

• Molecular Weight:

  19kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype:

  IgG