Recombinant Human Fibronectin

• Background :

  Fibronectin1 (FN1) is a secreted protein and contains 12 fibronectin type-I domains, fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II, and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.

• Product Name Alternative :

  FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1

• Accession Number :

  P02751

• Host :

  Human Cells

• Endotoxin :

  ≤10 EU/mg

• Purity :

  Greater than 95% as determined by reducing SDS-PAGE

• Form :

  Lyophilized

• Reconstitution :

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Molecular Weight :

  62.6 kDa

• Shipping Conditions :

  The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions :

  Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

• Formulation :

  Lyophilized From PBS,5% mannitol and 0.01% Tween 80, pH7.4

• Construct :

  Recombinant Human Fibronectin is produced by our Mammalian cell expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.

• Species :

  Human