APAF1 Rabbit mAb

• Background :

  APAF Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Induced by E2F and p53 in apoptotic neurons. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. 6 isoforms of the human protein are produced by alternative splicing.

• Description :

  This is a APAF1 Rabbit mAb Recombinant Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms :

  APAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413

• Swiss Prot :

  O14727

• Reactivity :

  Human, Mouse

• Immunogen :

  A synthesized peptide derived from human APAF1

• Clonality :

  Monoclonal

• Conjugation :

  Unconjugated

• Type :

  Recombinant Antibody

• Applications :

  WB, IHC, ICC/IF

• Purification Method :

  Affinity purification

• Assay Type :

  Antibody

• Dilution :

  WB 1:500-1:2000, IHC 1:50-1:200, ICC/IF 1:50-1:200

• Buffer :

  Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions :

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source :

  Rabbit

• Isotype :

  IgG