TFF-1

• Description:

  The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF1 is essential for normal differentiation of the antral and pyloric gastric mucosa, and functions as a gastric-specific tumor suppressor gene. Recombinant Murine TFF-1 is a 7.4 kDa monomeric protein consisting of a 66 amino acid polypeptide chain, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

• Synonyms:

  Trefoil Factor 1, pS2 protein, HP1.A, Breast Cancer Estrogen-Inducible protein (BCEI), PNR2

• NCBI Gene ID:

  21784

• UniProt:

  Q08423

• Accession Number:

  NP_033388.1

• Accession Number mRNA:

  NM_009362.2

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  QAQAQAQAQE ETCIMAPRER INCGFPGVTA QQCTERGCCF DDSVRGFPWC FHPMAIENTQ EEECPF

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Not available!

• Length:

  66

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  7.4 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source:

  E. coli