TACI / TNFRSF13B Protein (ECD, hFc Tag)

• Description:

  Tumor necrosis factor receptor superfamily, member 13B (TNFRSF13B) also known as Transmembrane activator and CAML interactor (TACI) and CD267 antigen, is a member of the tumor necrosis factor receptor superfamily. TNFRSF13B is a trimeric cytokine receptor that binds tumor necrosis factors (TNF) . The receptor cooperates with an adaptor protein which is important in determining the outcome of the response. Members of the TNF receptor superfamily (TNFRSF) have crucial roles in both innate and adaptive immunity and in cellular apoptosis process. Apoptosis is a cell suicide mechanism that enables metazoans to control cell number in tissues and to eliminate individual cells that threaten the animal's survival. Certain cells have unique sensors, termed death receptors or tumour necrosis factor (TNFR), on their surface. Tumour necrosis factors (TNFR) detect the presence of extracellular death signals and, in response, they rapidly ignite the cell's intrinsic apoptosis machinery. TACI/TNFRSF13B/CD267 induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand.

• Synonyms:

  CD267 Protein; CVID Protein; CVID2 Protein; IGAD2 Protein; RYZN Protein; TACI Protein; TNFRSF14B Protein

• Accession Number:

  NP_036584.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  HFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Bioactivity:

  1. Label Recombinant Human BLyS / TNFSF13B / BAFF Protein (Fc Tag) with biotin. Immobilized Recombinant Human TACI / TNFRSF13B Protein (ECD, Fc Tag) at 2 μg/mL (100 μL/well) can bind biotinylated Recombinant Human BLyS / TNFSF13B / BAFF Protein (Fc Tag) . The EC50 is 1.5-5.5 ng/mL. 2.Loaded Biotinylated Recombinant Human APRIL/TNFSF13 Protein, His & AVI Tag on SA Biosensor, can bind Recombinant Human TACI Protein, hFc Tag with an affinity constant of 39.200 nM as determined in BLI assay (Sartorius Octet RED384) .

• Length:

  403

• Form:

  Lyophilized

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  45 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells