NGF-beta

• Description:

  Β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues) . The three disulfide bonds are required for biological activity.

• Synonyms:

  NGF; NGFB; HSAN5; Beta-NGF

• NCBI Gene ID:

  4803

• UniProt:

  P01138

• Accession Number:

  NP_002497.2

• Accession Number mRNA:

  NM_002506

• Chromosomal Location:

  1p13.1

• Reactivity:

  Human

• Cross Reactivity:

  Chicken, Hamster, Mouse, Rat, Human, Leech

• Sequence:

  SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analysis

• Bioactivity:

  Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

• Length:

  240

• Form:

  Lyophilized

• Molecular Weight:

  13.5 kDa

• Host or Source:

  E. coli