Nesfatin

• Description :

  Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2) . Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic isletsβ-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.

• Synonyms :

  Nesfatin, Nucleobindin 2 protein

• NCBI Gene ID :

  4925

• UniProt :

  P80303

• Accession Number :

  NP_005004

• Accession Number mRNA :

  NM_005013.2

• Reactivity :

  Human

• Cross Reactivity :

  Human

• Sequence :

  VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 98% by SDS-PAGE & HPLC analysis

• Bioactivity :

  Determined by an in vivo assay using healthy wild type male mice (C57BL/6J) . Mice were treated via intraperitoneal injection once at a dose of 4μg Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.

• Length :

  82

• Form :

  Lyophilized

• Buffer :

  10mM Sodium Phosphate, pH 6.6

• Reconstitution :

  Water

• Molecular Weight :

  9.7 kDa

• Host or Source :

  E. coli