MIP-4 (CCL18)

• Description:

  MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4’s primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 68 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

• Synonyms:

  Macrophage Inflammatory Protein-4, CCL18, PARC, DC-CK1, AMAC-1

• NCBI Gene ID:

  6362

• UniProt:

  P55774

• Accession Number:

  NP_002979

• Accession Number mRNA:

  NM_002988

• Chromosomal Location:

  17q11.2

• Reactivity:

  Human

• Cross Reactivity:

  Human, Mouse, Bacteria

• Sequence:

  QVGTNKELC CLVYTSWQIP QKFLVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Assay #1: Determined by its ability to chemoattract human T lymphocytes using a concentration of 1.0 - 10.0 ng/ml. Assay #2: Determined by its ability to chemoattract human iDCs using a concentration of 1.0 - 10.0 ng/ml.

• Length:

  68

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  7.8 kDa

• Storage Conditions:

  For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % HSA or 0.1% BSA) and store in working aliquots at -20°C to -80°C. Lyophilized MIP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution MIP-4 can be stored at 4°C between 2-7 days and for future use below -20°C.

• Host or Source:

  E. coli