MIF

• Description:

  Macrophage Migration Inhibitory Factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through CD74 and CD44, resulting in the secretion of TNF-α, IL-1, IL-6, IL-8, and various MMPs. The enzymatic activity of MIF is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue. Insect cell derived recombinant MIF is a 15 kDa protein containing 124 amino acid residues, including an N-terminal His-tag.

• Synonyms:

  Macrophage migration Inhibitory factor, GLIF, MMIF, GIF, Glycosylation-inhibiting factor

• NCBI Gene ID:

  4282

• UniProt:

  P14174

• Accession Number:

  NP_002406.1

• Accession Number mRNA:

  NM_002415.1

• Chromosomal Location:

  22q11.23

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  His-Tag

• Sequence:

  HHHHHHHHAM PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to inhibit monocyte migration.

• Length:

  124

• Form:

  Lyophilized

• Buffer:

  10mM Sodium Citrate, pH 3.0

• Reconstitution:

  Water

• Molecular Weight:

  15 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source:

  Insect cells