Mesothelin Protein (His Tag)

• Description:

  The megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised of several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level) . It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.

• Synonyms:

  Mes Protein; MPF Protein; Msln Protein

• Accession Number:

  Q61468-1

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Label:

  His-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

• Bioactivity:

  Loaded anti-human MSLN antibody, IgG1 on ProA Biosensor, can bind Recombinant Mouse Mesothelin Protein, His Tag with an affinity constant of 0.14 μM as determined in BLI assay (Sartorius Octet RED384) .

• Length:

  314

• Form:

  Lyophilized

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  44 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells