MSLN / Mesothelin Protein (His Tag)

• Description:

  The megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised of several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level) . It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.

• Synonyms:

  CAK1 Protein; MPF Protein; SMRP Protein

• Accession Number:

  AAH09272.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  His-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

• Bioactivity:

  1.Loaded Anti-MSLN Antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human Mesothelin Protein, His Tag with an affinity constant of 0.965nM as determined in BLI assay (Sartorius Octet RED384) . 2.Immobilized Anti-MSLN Antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human MSLN / Mesothelin Protein (His Tag), the EC50 is 1.0-3.0 ng/mL.

• Length:

  296

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  34 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells