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Leptin, Pegylated

CAT: 0209-500-038-L100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0209-500-038-L100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mono-pegylated (with 20 kDa PEG) recombinant human leptin is an one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume recombinant mouse pegylated leptin runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 100 kDa protein. Recombinant human pegylated leptin half-life in circulation after SC injection was over 20 hours. Recombinant human leptin was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purification 47, 128–136 and then pegylated.
Synonyms
Lep; ob; obese
NCBI Gene ID
3952
UniProt
P41159
Accession Number
NP_000221.1
Accession Number mRNA
NM_000230
Reactivity
Human
Cross Reactivity
Human
Label
PEG
Sequence
AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC
Assay Protocol
It is recommended to reconstitute the lyophilized recombinant human pegylated leptin in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
Recombinant mouse pegylated leptin is capable of stimulatng proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 5-7 fold lower than the non-pegylated recombinant human leptin but in vivo it has profound weight reducing effect (as compared to the non-pegylated recombinant human leptin), resulting mainly from reduced food intake.
Length
146
Form
Lyophilized
Reconstitution
Water
Molecular Weight
35.6 kDa
Host or Source
E. coli
N Terminal Sequence
AVPIQ

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