IFN beta

• Description :

  Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFN-alpha is an acid stable molecule that signals through IFN-alpha/betaR, which is also used by IFN-beta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. Recombinant human IFN-beta is a 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFN-beta has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.

• Synonyms :

  IFNB1; IFB; IFF; IFNB

• NCBI Gene ID :

  3456

• UniProt :

  P01574

• Accession Number :

  NP_002167.1

• Accession Number mRNA :

  NM_002176

• Chromosomal Location :

  9p21

• Reactivity :

  Human

• Cross Reactivity :

  Monkey, Mouse, Human

• Sequence :

  MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity :

  Assay #1: Determined by its ability to induce STAT1/STAT2 activation in human COLO 205 ISRE LUC reporter cells. Assay #2: Determined by a cytotoxicity assay using human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml (specific activity of ≥ 1 x 10^7 units/mg) .

• Length :

  166

• Form :

  Lyophilized

• Molecular Weight :

  20 kDa

• Host or Source :

  CHO cells