GDF-3

• Description:

  GDF-3 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-9. Unlike most TGF-β family members, GDF-3 and GDF-9 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. The expression of GDF-3 is upregulated in high-fat-fed wild-type FABP4/aP2 null mice and was associated with obesity, but not with the related hyperglycemia/hyperinsulinemia which characterizes Type 2 diabetes. Recombinant human GDF-3 is a 26.0 kDa non-disulfide-linked homodimer containing two 114 amino acid polypeptide chains.

• Synonyms:

  GDF3; KFS3; MCOP7; MCOPCB6

• NCBI Gene ID:

  9573

• UniProt:

  Q9NR23

• Accession Number:

  NP_065685.1

• Accession Number mRNA:

  NM_020634

• Chromosomal Location:

  12p13.1

• Reactivity:

  Human

• Cross Reactivity:

  Mouse

• Sequence:

  AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 100-150 ng/ml.

• Length:

  114

• Form:

  Lyophilized

• Molecular Weight:

  26 kDa

• Host or Source:

  E. coli