GAcrp30 Variant

• Description:

  The gAcrp30 variant is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, Acrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs) . The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. This naturally occurring variant of human gAcrp30/Adipolean is an 18.1 kDa protein, containing 14 amino acids extra at the N-terminus of human gAcrp30/Adipolean.

• Synonyms:

  Apm-1 variant; Adiponectin

• NCBI Gene ID:

  9370

• UniProt:

  Q15848

• Accession Number:

  NP_004788.1

• Accession Number mRNA:

  NM_004797.3

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Rat, Human

• Sequence:

  PGAEGPRGFP GIQGRKGEPG EGAYVYRSAF SVGLETYVTI PNMPIRFTKI FYNQQNHYDG STGKFHCNIP GLYYFAYHIT VYMKDVKVSL FKKDKAMLFT YDQYQENNVD QASGSVLLHL EVGDQVWLQV YGEGERNGLY ADNDNDSTFT GFLLYHDTN

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by a cytotoxicity assay using M1 cells. The expected ED50 for this effect is 0.5-1.0 µg/ml.

• Length:

  159

• Form:

  Lyophilized

• Molecular Weight:

  18.1 kDa

• Host or Source:

  E. coli