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GAcrp30

CAT: 0209-M10-063-L100Size: 100 µgDry Ice: NoHazardous: No
CAT#:0209-M10-063-L100Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GAcrp30 is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs) . The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. Recombinant mouse gAcrp30 is a 16.6 kDa protein consisting of 145 amino acid residues.
Synonyms
Apm-1
NCBI Gene ID
11450
UniProt
Q60994
Accession Number
NP_033735.3
Accession Number mRNA
NM_009605.4
Reactivity
Mouse
Cross Reactivity
Mouse
Sequence
MKGEPGEAAY MYRSAFSVGL ETRVTVPNVP IRFTKIFYNQ QNHYDGSTGK FYCNIPGLYY FSYHITVYMK DVKVSLFKKD KAVLFTYDQY QEKNVDQASG SVLLHLEVGD QVWLQVYGDG DHNGLYADNV NDSTFTGFLL YHDTN
Assay Protocol
Centrifuge the vial prior to opening Reconstitute in 5mM Tris, pH 7.6 to 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to ‑80°C.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98% by SDS-PAGE & HPLC analyses
Bioactivity
Determined by its ability to inhibit the proliferation of murine myeloid cell lines M1. The ED50 for this effect is ≤ 15 µg/ml.
Length
145
Form
Lyophilized
Buffer
5mM Tris, pH 7.6
Reconstitution
5mM Tris, pH 7.6
Molecular Weight
16.6 kDa
Storage Conditions
The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
Host or Source
E. coli

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