FGF-2 (basic) heat stable, 154 aa

CAT: 0209-100-450-HS-L500Size: 500 µgDry Ice: NoHazardous: No
CAT#:0209-100-450-HS-L500Size:500 µg
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Description
FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic exerts proliferative activity towards a wide range of cell types and consequently is used as a vital component in numerous cell culture media formulations, including stem cell media. FGF-basic loses its activity over time in solution at physiological temperatures and needs to be resupplemented periodically to maintain its performance. As a result of selected amino acid substitutions, Heat Stable FGF-basic retains full biological activity and also maintains full activity over extended periods of time in solution at 37°C. These properties make Heat Stable FGF-basic a superior choice for use in cell culture media applications. Heat Stable Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acids.
Synonyms
Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin
NCBI Gene ID
2247
UniProt
P09038
Accession Number
NP_001997.5
Accession Number mRNA
NM_002006.4
Chromosomal Location
4q26
Reactivity
Human
Cross Reactivity
Human
Sequence
AAGSITTLPA LPEDGGSGAF PPGHFKDPKL LYCKNGGFFL RIHPDGRVDG TRDKSDPFIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL YAIKNVTDEC FFFERLEENN YNTYRSRKYP SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Assay Protocol
Centrifuge vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
≥ 95% by SDS-PAGE gel and HPLC analyses.
Bioactivity
Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is 0.05-0.25 ng/ml.
Length
154
Form
Lyophilized
Buffer
5mM Tris, pH 7.6 + 150mM NaCl
Reconstitution
5mM Tris, pH 7.6
Molecular Weight
17.2 kDa
Storage Conditions
The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.
Host or Source
E. coli

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