FAS Ligand, soluble

• Description:

  Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein (175 amino acid residues) comprising the TNF homologous region of FasL plus an eight His-Tag.

• Synonyms:

  FASLG; FASL; CD178; CD95L; CD95-L; TNFSF6; APT1LG1 S1697

• NCBI Gene ID:

  356

• UniProt:

  P48023

• Accession Number:

  NP_035254

• Accession Number mRNA:

  NM_000639.1

• Chromosomal Location:

  1q23

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Human

• Sequence:

  HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  >95% by SDS-PAGE & HPLC analysis

• Bioactivity:

  Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤ 10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.

• Length:

  156

• Form:

  Lyophilized

• Molecular Weight:

  17.9 kDa

• Host or Source:

  CHO cells