CNTF / Ciliary Neurotrophic Factor Protein (His Tag)

• Description:

  Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. Its actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracellular CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.

• Synonyms:

  Cntf Protein

• Accession Number:

  NP_740756.1

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Label:

  His-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Bioactivity:

  Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF at 10 μg/mL (100 μl/well) can bind biotinylated rat CNTFR-His. The EC50 of biotinylated rat CNTFR-His is 5-12 ng/mL.

• Length:

  215

• Form:

  Lyophilized

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  25 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  E.coli