CD86 / B7-2 Protein (His Tag)

• Description:

  CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

• Synonyms:

  B7-2 Protein; B7.2 Protein; B70 Protein; CD28LG2 Protein; LAB72 Protein

• Accession Number:

  NP_008820.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  His-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  > 97 % as determined by SDS-PAGE

• Bioactivity:

  1.Measured by its binding ability in a functional ELISA. Immobilized human CD86 at 20 μg/mL (100 μl/well) can bind human CD28 with a linear range of 32-800 ng/mL. 2.Loaded Recombinant Human B7-1 Protein on His1K Biosensor, can bind Recombinant Human CD28 Protein with an affinity constant of 0.46 µM as determined in BLI assay (Sartorius Octet RED384) . 3.Loaded Recombinant Human CD86 Protein on His1K Biosensor, can bind Recombinant Human CD28 Protein with an affinity constant of 2.72 μM as determined in BLI assay (Sartorius Octet RED384) .

• Length:

  231

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  57 kDa (glycosylated)

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells