C5a

CAT: 0209-100-056-L250Size: 250 µgDry Ice: NoHazardous: No
CAT#:0209-100-056-L250Size:250 µg
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Description
Complement 5a (C5a) is an enzymatically generated glycoprotein belonging to the anaphylatoxin family of structurally and functionally related proteins. Generated upon the activation of the complement system, C5a, together with C4a, C3a, and the membrane attack complex (C5b-9), functions as a central player in host defense by inducing smooth muscle cell contraction, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes through cell degranulation. In addition to acting as a direct mediator of localized inflammatory response, C5a also initiates both the synthesis and release of IL-8 from monocytes and bronchial epithelial cells, stimulates the proliferation of neurons and hepatocytes, and functions as a potent chemoattractant. Where C5a deficiency, a rare defect of the complement pathway caused by the mutation of the C5a gene, is associated with susceptibility to severe infections, excessive C5a activation has been linked to liver fibrosis, sepsis, adult respiratory distress syndrome, rheumatoid arthritis, Alzheimer’s disease, and ischemic heart disease. Human C5a shares 60% and 54% sequence identity to mouse and rat C5a, respectively. The human C5 gene encodes a 1,676 amino acid glycoprotein that is comprised of a disulfide-linked C5 alpha and a C5 beta chain, the former of which contains the active, 74 amino acid C5a anaphylatoxin chain. Recombinant Human C5a is an 8.28 kDa glycoprotein containing the 74 amino acid residues of the C5a anaphylatoxin chain.
Synonyms
Complement Component 5a, Complement 5a, CPAMD4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
NCBI Gene ID
727
UniProt
P01031
Accession Number
NP_001726.2
Accession Number mRNA
NM_001735.2
Reactivity
Human
Cross Reactivity
Human
Sequence
TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR
Assay Protocol
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at ‑20°C to -80°C.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
>98% by SDS-PAGE & HPLC analysis
Bioactivity
Measured by its ability to support adhesion of Human Umbilical Vein Endothelial Cells (HUVEC) to the Recombinant Human C5a-coated surface.
Length
74
Form
Lyophilized
Reconstitution
Water
Molecular Weight
8.3 kDa
Storage Conditions
The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
Host or Source
E. coli

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