BLyS / TNFSF13B / BAFF Protein (hFc Tag)

• Description:

  B lymphocyte stimulator (BLyS), also known as TNFSF13B, CD257 and BAFF, is a single-pass type II membrane protein, which belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFFR) . These receptors are a prominent factor in B cell differentiation, homeostasis, and selection. BLyS levels affect survival signals and selective apoptosis of autoantibody-producing B cells. Thus, it acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. Overexpression of BLyS in mice can lead to clinical and serological features of systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS) . BLyS is an attractive therapeutic target in human rheumatic diseases. The ability of BLyS to regulate both the size and repertoire of the peripheral B cell compartment raises the possibility that BLyS and antagonists thereof may form the basis of a therapeutic trichotomy. As an agonist, BLyS protein may enhance humoral immunity in congenital or acquired immunodeficiencies such as those resulting from viral infection or cancer therapy.

• Synonyms:

  BAFF Protein; BLYS Protein; CD257 Protein; DTL Protein; TALL-1 Protein; TALL1 Protein; THANK Protein; TNFSF20 Protein; TNLG7A Protein; UNQ401/PRO738 Protein; ZTNF4 Protein

• Accession Number:

  Q9Y275-1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  HFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  > 95 % as determined by SDS-PAGE

• Bioactivity:

  1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 1 μg/mL (100 μl/well) can bind human TNFRSF17. The EC50 of human TNFRSF17 is 0.07 μg/mL. 2. Measured in a cell proliferation assay in mouse splenocytes. The ED50 for this effect is typically 0.6-3.2 ng/mL. 3.Loaded Recombinant Human BAFF / BLyS / TNFSF13B Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag with an affinity constant of 0.267 μM as determined in BLI assay (Sartorius Octet RED384) . 4.Loaded Recombinant Human BAFF/BLyS/TNFSF13B Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag, Biotinylated with an affinity constant of 18.1 nM as determined in BLI assay (Sartorius Octet RED384) .

• Length:

  389

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet send with the product

• Molecular Weight:

  43.7 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells