Recombinant CD3D Monoclonal Antibody

• Background:

  CD3D is a component of the TCR-CD3 complex, which is present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition, CD3D plays an essential role in thymocyte differentiation. It participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. It interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.

• Abbreviation:

  CD3D

• UniProt:

  P04234

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human CD3D fragment

• Target:

  IMD; CD3D; CD3-DELTA; IMD19; T3D; T-Cell Receptor T3 Delta Chain; T-cell surface glycoprotein CD3 delta chain; T3; CD3 delta

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A525

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC; IF; FCM; IP

• Field of Research:

  Immunology; Stem Cells

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:2000-1:10000; IHC 1:200-1:1000; IF 1:50-1:200; FCM 1:50-1:100; IP 1:25-1:50

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  19 kDa

• Observed Molecular Weight:

  23 kDa

• Isotype:

  IgG, κ