Recombinant Aurora B Monoclonal Antibody

• Background:

  Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis.

• Abbreviation:

  Aurora B

• UniProt:

  Q96GD4

• Host:

  Rabbit

• Reactivity:

  Human; Mouse

• Immunogen:

  Recombinant human Aurora B fragment

• Target:

  IPL; ARK; AIK; AIRK; PPP1R; STK; AIM; AIK2; AIM-1; AIM1; ARK2; AurB; IPL1; PPP1R48; STK12; STK5; aurkb-sv1; aurkb-sv2; AURKB; Aurora B; AIRK2; STK1

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A136

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC; IF

• Field of Research:

  Cell Biology; Epigenetics and Nuclear Signaling; Signal Transduction; Cancer

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:1000; IHC 1:50-1:100; IF 1:50

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  39 kDa

• Observed Molecular Weight:

  39 kDa

• Isotype:

  IgG, κ