Recombinant Histone H4 (Acetyl Lys12) Monoclonal Antibody

• Background:

  Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5, 12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc.

• Abbreviation:

  Histone H4 (Acetyl Lys12)

• UniProt:

  P62805

• Host:

  Rabbit

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Acetylated human histone H4 (Lys12) peptide

• Target:

  H4F; methyl histone H; methylated histone H; HIST2H; HIST1H; HISTH4H; histone H; HIST2H4B; H4/o; histone H4; HIST1H4A; FO108; H4/p; HIST1H4; Hist4h4; HISTH4H4; methyl histone H4; methylated histone H4; H4/A; H4FA; H4/I; H4FI; HIST1H4B; H4/G; H4FG; HIST1H4C; H4/B; H4FB; HIST1H4D; H4/J; H4FJ; HIST1H4E; H4/C; H4FC; HIST1H4F; H4/H; H4FH; HIST1H4H; H4/M; H4FM; HIST1H4I; H4/E; H4FE; H4/D; H4FD; HIST1H4K; H4/K; H4FK; HIST1H4L; H4/N; H4F2; H4FN; HIST2H4; HIST2H4A; H4; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4-16; A; I; H4B; H4J; H4H; H4M; H4E; HIST1H4J; H4D; H4C; H4/p; histone H4; HIST1H4B H4/I

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A106

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC

• Field of Research:

  Epigenetics and Nuclear Signaling

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:1000; IHC 1:50-1:200

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  11 kDa

• Observed Molecular Weight:

  11 kDa

• Isotype:

  IgG, κ