Recombinant Phospho-eIF2α (Ser51) Monoclonal Antibody

• Background:

  The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]) .

• Abbreviation:

  EIF2α

• UniProt:

  P05198

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  A synthetic peptide corresponding to residues around (Ser51) of Human Phospho-eIF2α

• Target:

  EIF2S; EIF; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1; EIF 2; EIF 2 alpha; EIF 2A; EIF 2alpha; EIF2 alpha; eIF-2-alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit 1 alpha; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukaryotic translation initiation factor 2 subunit alpha; IF2A

• Clonality:

  Monoclonal, Recombinant

• Clone:

  12F6

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC

• Field of Research:

  Epigenetics and Nuclear Signaling

• Purification:

  Protein A

• Concentration:

  0.2 mg/mL

• Dilution:

  IHC 1:100-1:1000; WB 1:2000-1:10000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  36 kDa

• Observed Molecular Weight:

  36 kDa

• Isotype:

  IgG, κ